cd45.1 wt mice  (POSTECH Inc)

Journal: bioRxiv

Article Title: Enforced E-selectin ligand installation enhances homing and efficacy of adoptively transferred T cells

doi: 10.1101/2025.01.12.632650

Figure Lengend Snippet: (A) Experimental setup. (B) Tissue distribution of the adoptively transferred anti-hCEA mouse CAR-T cell at 24h after adoptive transfer (the percentages of control vs. fucosylated cells in total CAR-T cells in the tissue were shown). (C) Experimental setup and (D) flow cytometry analysis to distinguish transferred T cells that have entered tumor parenchyma from T cells in blood vasculature. (E) Distribution of fucosylated CAR-T cells in the MC38hCEA tumor analyzed by immunofluorescence, tumor #1, scale bar: 100 μm. In vivo antitumor efficacy (F) of CAR-T cells with or without fucosylation against MC38-hCEA colon cancer and mouse survival (G). 1×10 6 MC38hCEA tumor cells were subcutaneously injected into the C57BL/6J mice and 0.7×10 6 CAR-T cells were transferred into the recipient mice on day8 after tumor inoculation with lymphocyte depletion by irradiation with a dose of 5 Gy before CAR-T cell transfer. IV, intravenously injection. Grey arrows indicate the intraperitoneal anti-mouse IL-6 injection (150 ug/mouse). 5–6 repeats for each group (n=5–6), nsP > 0.05; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; survival curves were analyzed by log-rank test and other analysis used student T-test, and mean ±standard deviation (SD) values of biological replicates.

Article Snippet: C57BL/6J (both WT and CD45.1+/+ congenic strains) mice were purchased from Jackson Laboratory.

Techniques: Adoptive Transfer Assay, Control, Flow Cytometry, Immunofluorescence, In Vivo, Injection, Irradiation, Standard Deviation

Journal: bioRxiv

Article Title: Enforced E-selectin ligand installation enhances homing and efficacy of adoptively transferred T cells

doi: 10.1101/2025.01.12.632650

Figure Lengend Snippet: (A) Bone marrow metastasis was established by tail caudal artery injection, and control or fucosylated OT-I cells were transferred into metastasis bearing mice. (B) OT-I cell number in different organs at 24h after the cell transfer. (C) Control or fucosylated OT-I cells were transferred into the mice at 6–8h after challenged by E0771-OVA cells by IV injection and (D) OT-I cell number in different organs were analyzed at 24h after the cell transfer. (E-H) Cell-surface fucosylation improves CAR-T cell efficacy by enhancing T cell infiltration in E0771-E5 tumor. 1×10 6 E0771-E5 tumor cells were subcutaneously injected into the C57BL/6J mice and 1×10 6 E0771-E5 expressing luciferase were intravenously injected into the same mice. CAR-T cells were transferred into the mice on day8 after tumor injection with lymphocytes deletion by irradiation with a dose of 5 Gy before the CAR-T cell transfer. IV, intravenously injection. Lung metastasis was tracked by IVIS (E-H), tumor size (I), survival curves (J) and complete response (K) were recorded. 5–7 repeats for each group (n=5–7), nsP > 0.05; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; tumor size was analyzed by student T-test and survival curves were analyzed by log-rank test. Mean ±standard deviation (SD) values of biological replicates.

Article Snippet: C57BL/6J (both WT and CD45.1+/+ congenic strains) mice were purchased from Jackson Laboratory.

Techniques: Injection, Control, IV Injection, Expressing, Luciferase, Irradiation, Standard Deviation

Journal: bioRxiv

Article Title: Enforced E-selectin ligand installation enhances homing and efficacy of adoptively transferred T cells

doi: 10.1101/2025.01.12.632650

Figure Lengend Snippet: Reagents and antibodies

Article Snippet: C57BL/6J (both WT and CD45.1+/+ congenic strains) mice were purchased from Jackson Laboratory.

Techniques: Recombinant, Cell Isolation